Fluorescence Microscope
About
Fluorescence microscope Zeiss imager M2.m is a state of the art optical tool for morphology and fluorescence analysis.
Description
The device is a fluorescence microscope from Zeiss. Its main use is to characterize fluorescent markers or spots in microfluidic chips but is not limited to such. The microscope has an automated xyz-stage to obtain large area images or images with varying focus planes. Additionally standard white light microscopic tasks such as brightfield and darkfield imaging are possible.
Technical specifications:
- Colibri 7 LED light source for fluorescence excitation
- White light LED for prolonged lifetime and minimum energy consumption
- Imaging of fluorescent samples
- Automated xyz-stage
- Fully automated objective carousel (5x, 10x, 20x and 50x magnification)
- Brightfield/ Darkfield imaging
- Continuous imaging
- Large area characterization
- Z-axis plot for basic 2.5D characterization
- Option for surface characterization
Case study:
A die molecule is used as a gas detecting unit and is distributed in a matrix. To identify the distribution in the composite the fluorescence microscope is used and reveals a strong phase separation with some areas hosting a lot of die while other areas appear strongly depleted. From this follows that other fabrication routes need to be found to obtain a homogenous active sensing layer.
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